Journal: Aging
Article Title: WNT enhancing signals in pancreatic cancer are transmitted by LGR6.
doi: 10.18632/aging.205101
Figure Lengend Snippet: Figure 1. Baseline expression of LGR6 in PDAC cell lines. (A) qPCR of epithelial (CDH1), mesenchymal (ZEB1) markers, and LGR6. In addition, sphere-formation capacity (SFA) was evaluated. (B) Morphology of defined epithelial and mesenchymal PDAC cell lines; (C) Immunohistochemistry (left panels, Protein atlas) and immunofluorescence staining LGR6 (green) and E-cadherin (red) in Capan2; (D) Western blot analysis of LGR6 in pancreatic cancer cell lines. Quantification is shown as normalized to Capan2; (E) Flow cytometric analysis of extra- and intra-cellular LGR6 in pancreatic cancer cell lines; (F) Clustering analysis of the TCGA databank on PDAC identified LGR6 with epithelial marker group *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.
Article Snippet: Human pancreatic cancer cell lines (BxPC3, Capan2, MiaPaCa2, Panc1) were obtained from American Type Culture Collection (ATCC).
Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Staining, Western Blot, Marker